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antibodies
analysis of genes coding for antibodies
antibody affinity maturation
antibody engineering
bispecific antibody analysis
bispecific antibody production
bispecific antibody purification
coimmunoprecipitation (Co-IP)
competition ELISA
direct ELISA
dot blot
enzyme-Linked immunoSPOT (ELISPOT)
epitope binning
hybridoma generation
hybridoma screening
immunization (donkey)
immunization (horse)
immunization (llama)
immunization (mice)
immunization (rat)
indirect ELISA
inhibition ELISA
sandwich ELISA
Western Blot (WB)
aptamers
aptamer characterization
BN-PAGE
chemically-modified aptamers - practical knowledge
chemically-modified aptamers - theoretical knowledge
Click Chemistry reaction
electroelution of DNA
ELONA
HPLC
purification of modified nucleotides
SELEX
synthesis of modified nucleotides
whole-cell aptamer selection
baculovirus system
baculovirus titration
generation of recombinant baculoviruses
biophysical analysis
biolayer interferometry (BLI)
circular dichroism (CD)
confocal microscopy
differential scanning fluorimetry (DSF)
dynamic light scattering (DLS)
electroswitching biophysical measurement (switchSENSE technology)
fluorescence lifetime imaging (FLIM)
fluorescence microscopy
fluorescence resonance energy transfer (FRET)
fluorescence spectroscopy
immunefluorescence (IF)
isothermal titration calorimetry (ITC)
light microscopy
MALDI-TOF-MS
mass spectrometry
microscale thermophoresis (MST)
MS-based interactome identification
nuclear magnetic resonance (NMR)
scanning electron microscopy (SEM)
SEC-MALLS
surface plasmon resonance (SPR)
transmission electron microscopy (TEM)
widefield microscopy
cell biology
3D cell culture
adherent mammalian cell culture
biovisualisation
BrdU incorporation
cell banking
cell fractionation
cell imaging
cell invasion assays
cell migration assay
cells reprogramming
cryopreservation of cell lines
flow cytometry (cell cycle)
flow cytometry (multiple parameters)
flow cytometry (up to two parameters)
fluorescence in situ hybridization (FISH)
histopathology
human lymphocytes immortalization
IC50 analysis
immunocytochemistry (ICC)
immunohistochemistry (IHC)
isolation primary cells
lentiviral transfection
live-cell imaging
maintaining cell bank
mammalian cell viability assays
mammalian transfection
mouse lymphocytes immortalization
MTT
primary cell culture-based assays
primary mammalian cell culture
stable cell line generation
stem cell biology
suspension mammalian cell culture
work with a micromanipulator
work with stem cells
XTT
crystallography
designing crystalisation facility
managing crystallisation facility
membrane protein crystallography
optimization of crystallization conditions
protein crystallography
running crystalisation facility
sitting-drop format protein crystallization
vapor diffusion method protein crystallization
X-ray crystallography
microbiology
bacterial cell culture
bacterial culture
bacterial transformation
biofermentation
creating and monitoring the bank of microbial strains
filamentous fungi cell culture
identification of microorganisms
isolation of microorganisms
microbiology
performing environmental monitoring (air, clean room, staff, surface of devices)
performing of the antimicrobial effectiveness test
pharmaceutical microbiology
preparation of chemically competent bacterial cells
selection on agar plates
transfection of plant cells
transfection of yeast
validation of microbiological methods according to European Pharmacopoeia
yeast cell culture
yeast cell culture
yeast cell cycle analysis
yeast tetrad dissection
yeast transformation
yeast two hybrid
molecular biology
agarose electrophoresis
allelic discrimination
bacterial vectors
baculovirus expression vector system
Cre/Lox-P system
CRISPR/Cas9 system
DNA isolation
DNA methylation profiling
DNA mutagenesis (REMI)
DNA purification
DNA sequencing
electrophoretic mobility shift assay
electrophoretic mobility shift assay (EMSA)
error-prone mutagenesis
eukaryotic vectors design
Gateway cloning
gene expression analysis
gene overexpression
gene silencing
generation of fungal gene knock-outs
Gibson assembly
in-fusion cloning
mammalian vectors
microarrays
mono-cistronic cloning
multi-cistronic cloning
mutagenesis
nuclear export signals (NESs)
nuclear localization signals (NLSs)
nucleolus localization signals (NoLSs)
PCR
PCR optimization
PCR product purification
phage vectors
plasmid purification
primer design for cloning
primer design for qPCR
procaryotic vectors design
qPCR-HRM
real-time PCR
recombination system
reporter gene assays
restriction cloning
restriction-free cloning
RFLP
RNA isolation
RT-PCR
siRNA gene silencing
site-directed mutagenesis
Southern blot
synthetic biology
Synthetic Genetic Array
Tagouchi method for PCR optimization
yeast vectors
non-wetlab skills
bioinformatic analysis
bioIT analysis
change management
clinical trial design
convincing skills
customer care
data analysis
EU-funding aquisition
experiment design
experiment optimization
experiment troubleshooting
FloJo
Flowing Software
Geneious
GraphPad Prism
in silico analysis of nucleic acids using bioinformatics tools
in silico analysis of proteins using bioinformatics tools
knowledge in early drug development stages
knowledge in late drug development stages (clinical)
leadership skills
management certification
management of human resources
MBA
MySQL
NCBI databases searching
negotiating
Office package
organization management
partner search
patent search (advanced)
patent search (basic)
patent translation (ENG to PL)
patent translation (PL to ENG)
persuading
preclinical trial design
project management
protocol optimization
protocol validation
R
regulatory affairs
SnapGene
Statista
statistical analysis
statistical analysis
team management
time management
tutoring experience
writing of grant proposals
writing of reports
writing of scientific manuscripts
phage display
counterselection in phage display selection
donor-based library generation
filamentous phage amplification
filamentous phage purification
filamentous phage titration (plaques)
filamentous phage titration (cfu)
Kunkel mutagenesis
monoclonal phage ELISA (library screening)
NGS - data analysis
NGS - material preparation
phage display selection on magnetic beads
phage display selection on MaxiSorp plates
phage display selection on whole cells
polyclonal phage ELISA
soluble fragment ELISA (library screening)
synthetic library design (randomization scheme)
synthetic library generation
plant science
ODN plant genetic modification
plant agrotransformation
plant biolistic transformation
plant passage
seed passage
seeds sterilization
work with plant material
protein chemistry
acyl-RAC assay
AKTA system
blue native polyacrylamide gel electrophoresis (BN-PAGE)
Bradford standard curve preparation
enzymatic assay development
enzymatic assay optimization
Far Western blotting
fluorescent modification of proteins
FPLC system
HIC
His-tagged protein purification
IEC
IEX
IMAC
immunoglobulin purification
intrinsically disordered proteins (IDPs)
isoelectric focusing
native polyacrylamide gel electrophoresis (native PAGE)
Phos-tag technology for phosphorylated proteins
preparation of buffers and solutions
preparation of SDS-PAGE gels
protein acylation assay
protein biotinylation
protein concentration analysis
protein concentrations (A280)
protein concentrations (BCA)
protein concentrations (Bradford)
protein dialysis
protein engineering
protein expression in bacterial system
protein expression in cell-free system
protein expression in insect system
protein expression in mammalian system
protein formulation studies
protein PEGylation
protein purification from bacterial system
protein purification from inclusion bodies
protein purification from mammalian system
protein refolding
protein stability tests
recombinant antibody production in mammalian cells
SEC
sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
two-dimension blue native polyacrylamide gel electrophoresis (2D-BN-PAGE)
two-dimension polyacrylamide gel electrophoresis (2D-PAGE)
untagged protein purification
UPLC system
quality control
calibration of laboratory equipment
clean room (designing)
clean room (knowledge about standards)
clean room (working in)
cosmetic brand launching
cosmetic ingredient (making)
CPNP - Cosmetic Products Notification Portal
environmental monitoring (air, clean room, surface of devices)
GLP (auditor)
GLP (internal auditor)
GLP (working in compliant lab)
GMP (auditor)
GMP (internal auditor)
GMP (working in compliant lab)
good clinical practice (GCP)
ISO13485 (auditor)
ISO13485 (internal auditor)
ISO13485 (working in the compliant lab)
ISO17025 (auditor)
ISO17025 (internal auditor)
ISO17025 (working in the compliant lab)
ISO22716 (auditor)
ISO22716 (internal auditor)
ISO22716 (working in the compliant lab)
ISO9001 (auditor)
ISO9001 (internal auditor)
ISO9001 (working in the compliant lab)
laboratory stock control
maintenance of laboratory equipment
product packaging design
quality assurance system
quality management system
reagent ordering system - management
validation of laboratory equipment
other
antioxidant capacity analysis
aseptic technique
ATP measurement
bimolecular fluorescence complementation (BiFC)
cellular protein trafficking
chemical crosslinking
cholesterol assay
colorimetric assays
D509
EVs (extrachromosomal vesicles) isolation
fluorimetric assays
glicoprofiling
glycan analysis
glycobiology
GSEA analysis
GUV liposome preparation
handling laboratory animals (certified)
handling laboratory animals (uncertified)
isolation of cellular structures
lipid-protein interactions (Langmuir-Blodgett technique)
liposome technology
LUV liposome preparation
membrane fluidity assays
metabolite analysis (UPLC-MS)
metabolites measurement
organelle purification
phosphate assay
processing and analysis of patients samples
RP-HPLC
VNTR analysis
work with cross-linked polyethylene (PEX)
working with biofluids
working with solid tissues
zymography
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